平闆計數瓊脂 PCA 培養基的配方[ 青島日水 ]

平闆計數瓊脂（PCA）培養基的配方[ 青島日水 ]

用途：用於細菌總數測定（GB 4789.2-2010，SN0168-92 ，GB13093-91，SN/T 2098-2008和ISO4833-2003）。

原理：蛋白胨和酵母浸粉作爲基礎營養物質提供一般細菌生長所需的氮源、碳源、B族維生素和其它營養物質；葡萄糖提供生長代謝所需的能量物質；瓊脂作爲凝固劑。

配製方法:

稱取本品23.5g於1L蒸餾水或去離子水中（可按比例增加或減少配製量）,加熱煮沸至完全溶解,分裝,121℃髙壓滅菌15分鍾,滅菌結束後請搖勻,以防瓊脂沉積於器皿底部而凝固,備用。

Formula(配方)： g/L

Tryptone（胰蛋白胨）：5.0g

Yeast Extract（酵母浸粉）：2.5g

Dextrose（葡萄糖）：1.0g

Agar（瓊脂）：15.0g

pH7.0±0.2(25℃)

Formulation of Plate Count Agar (PCA) medium

Usage: Used for total bacterial count determination (GB 4789.2-2010, SN0168-92, GB13093-91, SN/T 2098-2008 and ISO4833-2003).

Principle: peptone and yeast extract as basic nutrients to provide nitrogen, carbon, B vitamins and other nutrients for general bacterial growth; glucose to provide the energy needed for growth and metabolism; agar as a coagulant.

Method of preparation:

Weigh this product 23.5g in 1L distilled water or deionized water (can be proportionally increased or reduced formulation), heat and boil until completely dissolved, sub-packed, high-pressure sterilization at 121 C for 15 minutes, shake well after sterilization, in order to prevent agar deposition in the bottom of the vessel and solidify, reserve.

Formula (formula):g/L

Tryptone (tryptone) 5.0g

Yeast Extract (yeast extract powder) 2.5G

Dextrose (glucose) 1.0g

Agar (agar) 15.0g

PH7.0 + 0.2 (25 C)

青島日水生物技術有限公司
山東省青島市黃島區黃河西路86號

www.qdrishui.cn

聯繫人信息